September 2018
Sensors and Actuators B: Chemical
Multiple optical trapping assisted bead-array based fluorescence assay of free and total prostate-specific antigen in serum
Camera used: Evolve 512 EMCCD
August 2018
Bioelectrochemistry
Plasma membrane depolarization and permeabilization due to electric pulses in cell lines of different excitability
Camera used: Evolve 512 EMCCD
Cellular Signalling
The cAMP-induced G protein subunits dissociation monitored in live Dictyostelium cells by BRET reveals two activation rates, a positive effect of caffeine and potential role of microtubules
Camera used: Evolve 512 EMCCD
July 2018
bioRxiv
Super-resolution fight club: A broad assessment of 2D & 3D single-molecule localization microscopy software
Camera used: Evolve 512 Delta EMCCD
Cell Reports
MTSS1 Regulation of Actin-Nucleating Formin DAAM1 in Dendritic Filopodia Determines Final Dendritic Configuration of Purkinje Cells
Camera used: Evolve 512 EMCCD
Current Biology
Activity-Dependent Actin Remodeling at the Base of Dendritic Spines Promotes Microtubule Entry
Camera used: Evolve 512 EMCCD
Developmental Cell
Local Arrangement of Fibronectin by Myofibroblasts Governs Peripheral Nuclear Positioning in Muscle Cells
Camera used: Evolve 512 EMCCD
Developmental Cell
CLASP Suppresses Microtubule Catastrophes through a Single TOG Domain
Camera used: Evolve 512 Delta EMCCD CoolSNAP MYO
Human Molecular Genetics
Inositol trisphosphate receptor-mediated Ca2+ signalling stimulates mitochondrial function and gene expression in core myopathy patients
Camera used: Evolve 512 Delta EMCCD
Human Molecular Genetics
Mutation of FOP/FGFR1OP in mice recapitulates human short rib-polydactyly ciliopathy
Camera used: Evolve 512 EMCCD
Journal of Cell Biology
Dynamics of RecA-mediated repair of replication-dependent DNA breaks
Camera used: Evolve 512 EMCCD
Journal of Medical Chemistry
A New Fluorogenic Small-Molecule Labeling Tool for Surface Diffusion Analysis and Advanced Fluorescence Imaging of β-Site Amyloid Precursor Protein-Cleaving Enzyme 1 Based on Silicone Rhodamine: SiR-BACE1
Camera used: Evolve 512 Delta EMCCD
Journal of Virology
Truncated CPSF6 Forms Higher-Order Complexes That Bind and Disrupt HIV-1 Capsid
Camera used: Prime 95B Scientific CMOS
Mucosalimmunology
Herpes simplex virus-binding IgG traps HSV in human cervicovaginal mucus across the menstrual cycle and diverse vaginal microbial composition
Camera used: Evolve 512 EMCCD
The Plant Journal
A conserved RxLR effector interacts with host RABA-type GTPases to inhibit vesicle-mediated secretion of antimicrobial proteins
Camera used: Evolve 512 EMCCD
June 2018
Sensors and Actuators B: Chemical
Precision guided microfluidic chemical perfusion strategy for cell dynamic imaging
Camera used: Evolve 512 EMCCD
ACS Chemical Neuroscience
Measurement of Tau Filament Fragmentation Provides Insights into Prion-like Spreading
Camera used: Evolve 512 Delta EMCCD
ACS Chemical Biology.
Multistep Inhibition of α-Synuclein Aggregation and Toxicity in Vitro and in Vivo by Trodusquemine
Camera used: Evolve 512 EMCCD
BBA Molecular Cell Research
Dynamic L-type CaV1.2 channel trafficking facilitates CaV1.2 clustering and cooperative gating
Camera used: Prime 95B Scientific CMOS
bioRxiv
Soluble CD93 is an Apoptotic Cell Opsonin Recognized by the αxβ2 Integrin
Camera used: Evolve 512 EMCCD
bioRxiv
Is Actin Filament Sliding Responsible for Endoplasmic Reticulum and Golgi Movement?
Camera used: Prime 95B Scientific CMOS
bioRxiv
Exocyst Dynamics During Vesicle Tethering and Fusion
Camera used: Prime 95B Scientific CMOS
Cell Reports
Single-Molecule Characterization of the Interactions between Extracellular Chaperones and Toxic α-Synuclein Oligomers
Camera used: Evolve 512 EMCCD
Cellular Microbiology
The surreptitious survival of the emerging pathogen Staphylococcus lugdunensis within macrophages as an immune evasion strategy
Camera used: Evolve 512 EMCCD
Chemical Science
Dynamically imaging collision electrochemistry of single electrochemiluminescence nano-emitters
Camera used: Evolve 512 Delta EMCCD
Texas Tech University Library
VMMO Lunar Volatile and Mineralogy Mapping Orbiter
Camera used: Prime 95B Scientific CMOS
eLIFE
Kinetics of HIV-1 capsid uncoating revealed by single-molecule analysis
Camera used: Evolve 512 EMCCD
EMBO Reports
Adducin-1 is essential for spindle pole integrity through its interaction with TPX2
Camera used: Evolve 512 Delta EMCCD
International Journal of Pharmaceutics
Pseudomonas aeruginosa pyocyanin production reduced by quorum-sensing inhibiting nanocarriers
Camera used: Evolve 512 EMCCD
Journal of Cell Biology
Myosin VI and branched actin filaments mediate membrane constriction and fission of melanosomal tubule carriers
Camera used: Prime 95B Scientific CMOS
The Journal of Physiology
Transverse cardiac slicing and optical imaging for analysis of transmural gradients in membrane potential and Ca2+ transients in murine heart
Camera used: Evolve 128 EMCCD
Journal of Virology
Persistent Replication of a Chikungunya Virus Replicon in Human Cells is Associated with Presence of Stable Cytoplasmic Granules Containing Non-structural Protein 3
Camera used: Prime 95B Scientific CMOS
MBio
The Pearling Transition Provides Evidence of Force-Driven Endosomal Tubulation during Salmonella Infection
Camera used: Prime 95B Scientific CMOS
Molecular Therapy
RTN4 Knockdown Dysregulates the AKT Pathway, Destabilizes the Cytoskeleton, and Enhances Paclitaxel-Induced Cytotoxicity in Cancers
Camera used: Evolve 512 EMCCD
Nature Communications
α-synuclein oligomers interact with ATP synthase and open the permeability transition pore in Parkinson’s disease
Camera used: Evolve 512 EMCCD
Nature Communications
FRET-enhanced photostability allows improved single-molecule tracking of proteins and protein complexes in live mammalian cells
Camera used: Evolve 512 EMCCD Evolve 512 Delta EMCCD
Nucleic Acids Researcj
RecBCD coordinates repair of two ends at a DNA double-strand break, preventing aberrant chromosome amplification
Camera used: Evolve 512 EMCCD
PLoS Genetics
Calcium and cAMP directly modulate the speed of the Drosophila circadian clock
Camera used: Evolve 512 EMCCD
PLoS Genetics
A neuronal MAP kinase constrains growth of a Caenorhabditis elegans sensory dendrite throughout the life of the organism
Camera used: Evolve 512 Delta EMCCD CoolSNAP HQ2
PNAS
RalA controls glucose homeostasis by regulating glucose uptake in brown fat
Camera used: Evolve 512 EMCCD
RSC Advances
Amplifying fluorescence signal contrast of aptamer-modified microspheres inspired by whispering-gallery mode lasing
Camera used: Evolve 512 EMCCD
Science Signaling
The channel-kinase TRPM7 regulates antigen gathering and internalization in B cells
Camera used: Evolve 512 Delta EMCCD
Stem Cell Reports
Human Induced Pluripotent Stem Cell-Derived Cardiac Cell Sheets Expressing Genetically Encoded Voltage Indicator for Pharmacological and Arrhythmia Studies
Camera used: Evolve 512 Delta EMCCD
Wellcome Open Research
Probing Plasmodium falciparum sexual commitment at the single-cell level
Camera used: Evolve 512 Delta EMCCD
May 2018
Biology Open
Spindly is required for rapid migration of human cells
Camera used: Evolve 512 Delta EMCCD
bioRxiv
An ancestral role of pericentrin in centriole formation through SAS-6 recruitment
Camera used: Evolve 512 EMCCD
bioRxiv
Optogenetic dissection of mitotic spindle positioning in vivo
Camera used: Evolve 512 EMCCD
bioRxiv
The RhoGAP myosin 9/HUM-7 integrates membrane signals to modulate Rho/RHO-1 during embryonic morphogenesis in C. elegans
Camera used: Evolve 512 EMCCD
bioRxiv
Protein Gradients on the Nucleoid Position the Carbon-fixing Organelles of Cyanobacteria
Camera used: Prime 95B Scientific CMOS
bioRxiv
Aggregation of Vibrio cholerae by cationic polymers enhances quorum sensing but over-rides biofilm dissipation in response to autoinduction
Camera used: Evolve 512 EMCCD
Cell Death & Differentiation
MICU3 is a tissue-specific enhancer of mitochondrial calcium uptake
Camera used: Evolve 512 EMCCD
Cell Reports
Nup133 Is Required for Proper Nuclear Pore Basket Assembly and Dynamics in Embryonic Stem Cells
Camera used: Prime 95B Scientific CMOS Evolve 512 EMCCD
ournal of Cell Science
Lysosome enlargement during inhibition of the lipid kinase PIKfyve proceeds through lysosome coalescence
Camera used: Prime 95B Scientific CMOS
MBoC (Molecular Biology of the Cell)
Myosin-dependent actin stabilization as revealed by single-molecule imaging of actin turnover
Camera used: Evolve 512 EMCCD
Nature Communications
Single quantum dot tracking reveals the impact of nanoparticle surface on intracellular state
Camera used: Evolve 512 EMCCD
Nature Communications
Super-resolution architecture of mammalian centriole distal appendages reveals distinct blade and matrix functional components
Camera used: Evolve 512 Delta EMCCD
Nature Communications
Live-cell single-molecule dynamics of PcG proteins imposed by the DIPG H3.3K27M mutation
Camera used: Evolve 512 EMCCD
Nature Protocols
Combining fluorescence imaging with Hi-C to study 3D genome architecture of the same single cell
Camera used: Evolve 512 EMCCD
Nature Structural & Molecular Biology
Conformational plasticity in the selectivity filter of the TRPV2 ion channel
Camera used: Prime 95B Scientific CMOS
Optics Express
Integrated one- and two-photon scanned oblique plane illumination (SOPi) microscopy for rapid volumetric imaging
Camera used: Prime 95B Scientific CMOS
Physical Review Letters
Motion of Knots in DNA Stretched by Elongational Fields
Camera used: Prime 95B Scientific CMOS
PLoS ONE
Directed fusion of cardiac spheroids into larger heterocellular microtissues enables investigation of cardiac action potential propagation via cardiac fibroblasts
Camera used: Evolve 128 EMCCD
PNAS
Spatial and temporal organization of cadherin in punctate adherens junctions
Camera used: Prime 95B Scientific CMOS
April 2018
Biochimica Biophysica Acta
Cardiolipin synthesizing enzymes form a complex that interacts with cardiolipin-dependent membrane organizing proteins
Camera used: Evolve 512 EMCCD
Experimental Eye Research
Reduced intraepithelial corneal nerve density and sensitivity accompany desiccating stress and aging in C57BL/6 mice
Camera used: Evolve 512 Delta EMCCD
bioRxiv
The RZZ complex integrates spindle checkpoint maintenance with dynamic expansion of unattached kinetochores
Camera used: Evolve 512 EMCCD
bioRxiv
CD161 mediates prenatal immune suppression of IFNγ-producing PLZF+ T cells
Camera used: Prime 95B Scientific CMOS
bioRxiv
TSA-Seq Mapping of Nuclear Genome Organization
Camera used: Evolve 512 EMCCD
bioRxiv
A cell separation checkpoint that enforces the proper order of late cytokinetic events
Camera used: Evolve 512 EMCCD
Scientific Reports
Switching between individual and collective motility in B lymphocytes is controlled by cell-matrix adhesion and inter-cellular interactions
Camera used: Evolve 512 EMCCD
Scientific Reports
Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system
Camera used: Evolve 512 EMCCD
Amerian Journal of Physiology-Cell Physiolog
Lipidated connexin mimetic peptides potently inhibit gap junction-mediated Ca2+-wave propagation
Camera used: Evolve 512 EMCCD
Cell
FUS Phase Separation Is Modulated by a Molecular Chaperone and Methylation of Arginine Cation-π Interactions
Camera used: Evolve 512 EMCCD
PLoS Genetics
Phosphorelay through the bifunctional phosphotransferase PhyT controls the general stress response in an alphaproteobacterium
Camera used: Evolve 512 EMCCD
Theranostics
Polypyrrole-chitosan conductive biomaterial synchronizes cardiomyocyte contraction and improves myocardial electrical impulse propagation
Camera used: Evolve 128 EMCCD
March 2018
Nature Communications
Over-elongation of centrioles in cancer promotes centriole amplification and chromosome missegregation
Camera used: Evolve 512
Biophotonics Congress: Biomedical Optics Congress 2018 (Microscopy/Translational/Brain/OTS)
Nanoscale Imaging of Chromatin with Labeled and Label-Free Super-Resolution Microscopy and Partial-Wave Spectroscopy
Camera used: Prime 95B
Journal of Cell Biology
TRRAP is a central regulator of human multiciliated cell formation
Camera used: Evolve 512
ACS Chemical Neuroscience
CB1 Cannabinoid Receptor Expression in the Barrel Field Region Is Associated with Mouse Learning
Camera used: Evolve 512
Current Genetics
Induction and relocalization of telomeric repeat-containing RNAs during diauxic shift in budding yeast
Camera used: Evolve 512
Journal of Controlled Release
Quantification of ligand density and stoichiometry on the surface of liposomes using single-molecule fluorescence imaging
Camera used: Evolve 512
Journal of General Physiology
Dynamic rearrangement of the intrinsic ligand regulates KCNH potassium channels
Camera used: Evolve 512
bioRxiv
Microtubule end tethering of a processive Kinesin-8 motor Kif18b is required for spindle positioning
Camera used: Evolve 512 Delta
Photon Counting – Fundamentals and Applications
Detectors for Super-Resolution & Single-Molecule Fluorescence Microscopies
Camera used: Evolve 128 Evolve 512 Prime CoolSNAP DYNO
European Journal of Pharmaceutical Sciences
Effects of enzymatic degradation on dynamic mechanical properties of the vitreous and intravitreal nanoparticle mobility
Camera used: Evolve 512 EMCCD
Structure
Structure-Function Relationship of the Bik1-Bim1 Complex
Camera used: Evolve 512 EMCCD
Molecular Biology of the Cell
Control of septin filament flexibility and bundling by subunit composition and nucleotide interactions
Camera used: Prime 95B Scientific CMOS
Journal of Cell Biology
Motor axon navigation relies on Fidgetin-like 1-driven microtubule plus end dynamics
Camera used: Evolve 512 EMCCD
PLoS ONE
RAGE-specific single chain Fv for PET imaging of pancreatic cancer
Camera used: Evolve 512 Delta EMCCD
bioRxiv
3D micro-environment regulates NF-κβ dependent adhesion to induce monocyte differentiation
Camera used: Evolve 512 Delta EMCCD
Autophagy
TP53 is required for BECN1- and ATG5-dependent cell death induced by sphingosine kinase 1 inhibition
Camera used: Evolve 512 EMCCD
Cell Death & Differentiation
Extracellular anti-angiogenic proteins augment an endosomal protein trafficking pathway to reach mitochondria and execute apoptosis in HUVECs
Camera used: Prime 95B Scientific CMOS Evolve 512 EMCCD
Neuron
Caldendrin Directly Couples Postsynaptic Calcium Signals to Actin Remodeling in Dendritic Spines
Camera used: Evolve 512 EMCCD
Analytical Chemistry
Microfluidic Diffusion Platform for Characterizing the Sizes of Lipid Vesicles and the Thermodynamics of Protein-Lipid Interactions
Camera used: Evolve 512 EMCCD
Journal of Neuroscience
β-Secretase BACE1 promotes surface expression and function of Kv3.4 at hippocampal mossy fiber synapses
Camera used: Evolve 512 EMCCD
Molecular Cell
The Ubiquitin E3/E4 Ligase UBE4A Adjusts Protein Ubiquitylation and Accumulation at Sites of DNA Damage, Facilitating Double-Strand Break Repair
Camera used: Evolve 512 EMCCD
Nature Genetics
A CRISPR-based screen for Hedgehog signaling provides insights into ciliary function and ciliopathies
Camera used: Prime 95B Scientific CMOS
bioRxiv
GPRIN1 modulates neuronal signal transduction and affects mouse-learning behavior
Camera used: Evolve 512 EMCCD
Nature Communications
RodZ modulates geometric localization of the bacterial actin MreB to regulate cell shape
Camera used: Evolve 512 EMCCD
PLoS ONE
Administration of growth factors promotes salisphere formation from irradiated parotid salivary glands
Camera used: Evolve 512 EMCCD
February 2018
ACS Central Science
Conditional and Reversible Activation of Class A and B G Protein-Coupled Receptors Using Tethered Pharmacology
Camera used: Evolve 512 Delta
Soft Matter
Knots modify the coil-stretch transition in linear DNA polymers
Camera used: Prime 95B
Nature Communications
Membrane cholesterol mediates the cellular effects of monolayer graphene substrates
Camera used: Evolve 512
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV
Correlative FRET: new method improves rigor and reproducibility in determining distances within synaptic nanoscale architecture
Camera used: Prime 95B
Langmuir
Direct Mapping of Heterogeneous Surface Coverage in DNA-Functionalized Gold Surfaces with Correlated Electron and Fluorescence Microscopy
Camera used: Evolve 512
Scientific Reports
VPS4 is a dynamic component of the centrosome that regulates centrosome localization of γ-tubulin, centriolar satellite stability and ciliogenesis
Camera used: Evolve 512
bioRxiv
Control of flagellum length by a grow-and-lock model
Camera used: Evolve 512
Molecular Cell
Mitochondrial MDM2 Regulates Respiratory Complex I Activity Independently of p53
Camera used: Prime 95B
EMBO J
Regulation of Hed1 and Rad54 binding during maturation of the meiosis-specific presynaptic complex
Camera used: Evolve 512
Nano Letters
Subcellular Nanorheology Reveals Lysosomal Viscosity as a Reporter for Lysosomal Storage Diseases
Camera used: Evolve 512 Delta
Nature Communications
Detecting RNA base methylations in single cells by in situ hybridization
Camera used: Evolve 512
bioRxiv
Cholesterol depletion by MβCD enhances membrane tension, its heterogeneity and affects cellular integrity.
Camera used: Evolve 512 Delta
BMC Cancer
Plocabulin, a novel tubulin-binding agent, inhibits angiogenesis by modulation of microtubule dynamics in endothelial cells
Camera used: Evolve 512
Langmuir
On the Complex and Reversible Pathways of CdSe Quantum Dots Driven by Pyroelectric-Dielectrophoresis
Camera used: Evolve 512
Development
The elmo-mbc complex and rhogap19d couple Rho family GTPases during mesenchymal-to-epithelial-like transitions
Camera used: Evolve 512
Methods in Molecular Biology
Single-Particle Tracking of Cell Surface Proteins
Camera used: Evolve 512 Delta
Nature Communications
Tuning microtubule dynamics to enhance cancer therapy by modulating FER-mediated CRMP2 phosphorylation
Camera used: Evolve 512 Delta
Advanced Engineering Materials
Multi-Stable Mechanical Structural Materials
Camera used: Evolve 128
Royal Society Open Science
Bifunctional fluorescent probes for detection of amyloid aggregates and reactive oxygen species
Camera used: Evolve 512 Delta
January 2018
Journal of Modern Optics
Enhancing the imaging quality and fabrication efficiency of bionic compound eyes using a sandwich structure
Camera used: Evolve 128
Amyotrophic Lateral Sclerosis and Frontotemporal Degeneration
Primary fibroblasts derived from sporadic amyotrophic lateral sclerosis patients do not show ALS cytological lesions
Camera used: Evolve 512
bioRxiv
In vivo optochemical control of cell contractility at single cell resolution by Ca2+ induced myosin activation
Camera used: Evolve 512
bioRxiv
Single-molecule dynamics and genome-wide transcriptomics reveal that NF-kB (p65)-DNA binding times can be decoupled from transcriptional activation
Camera used: Evolve 512
bioRxiv
A genetically encoded biosensor reveals location bias of opioid drug action
Camera used: Evolve 512 Delta
Frontiers in Physiology
Sympathetic Nervous Regulation of Calcium and Action Potential Alternans in the Intact Heart
Camera used: Evolve 512
Nature Communications
Active and dynamic mitochondrial S-depalmitoylation revealed by targeted fluorescent probes
Camera used: Evolve 512 Delta
Bio-Protocol
Measuring Mitochondrial ROS in Mammalian Cells with a Genetically Encoded Protein Sensor
Camera used: Evolve 512
bioRxiv
Lattice defects induce microtubule self-renewal
Camera used: Evolve 512
bioRxiv
Spindly is Required for Rapid Migration of Human Cells.
Camera used: Evolve 512 Delta
bioRxiv
Coupling of Rho family GTPases during mesenchymal-to-epithelial-like transitions
Camera used: Evolve 512
Bio Protoc
Real-time Analysis of Auxin Response, Cell Wall pH and Elongation inArabidopsis thalianaHypocotyls
Camera used: Evolve 512
Plant, Cell & Environment
Entrainment of Arabidopsis roots to the light:dark cycle by light piping
Camera used: Evolve 512
bioRxiv
Dynamin-2 facilitates Atg9 recycling from nascent autophagosomes
Camera used: Evolve 512