| Area | Lumencor Light Engine | Application | Reference | Authors | PMID | Year |
|---|---|---|---|---|---|---|
| Bioengineering | SPECTRA X | Optogenetic manipulation of cyclic dimeric guanosine monophosphate (c-di-GMP) levels in Pseudomonas aeruginosa biofilms | Bioprinting living biofilms through optogenetic manipulation. ACS Synth Biol (2018) 7(5):1195–1200 | Y Huang, A Xia, F Jin et al. | 29664610 | 2018 |
| Bioengineering | SOLA SE | Time lapse imaging of promoter activation and plasmid copy number in transformed E. coli. | Synchronized DNA cycling across a bacterial population. Nat Genet (2017) 49(8):1282–1285 | L Baumgart, W Mather, J Hasty | 28692067 | 2017 |
| Bioengineering | SOLA SE | Excitation of CellTracker Blue CMAC and GFP for image-based determination of plasmid transfection versus cell number | Plasmid transfection in bovine cells: Optimization using a realtime monitoring of green fluorescent protein and effect on gene reporter assay. Gene (2017) 626:200–208 | JS Osorio, M Bionaz | 28501631 | 2017 |
| Bioengineering | SOLA SE | Excitation of autofluorescence for time-lapse imaging of cyanobacteria and algae. | A microfluidic platform for long-term monitoring of algae in a dynamic environment. ACS Synth Biol (2016) 5(1):8–14 | CS Luke, J. Selimkhanov, J Hasty et al. | 26332284 | 2016 |
| Bioengineering | SOLA SE | Imaging of GFP and RFP expression for monitoring gene deletion in E. coli | Targeted large-scale deletion of bacterial genomes using CRISPR-nickases. ACS Synth Biol (2015) 4(11):1217–1225 | K Standage-Beier, Q Zhang, X Wang | 26451892 | 2015 |
| Confocal Microscopy | SPECTRA X | Excitation of mCherry for live cell confocal imaging using X-Light spinning disk system | In vivo imaging of DNA double-strand break induced telomere mobility during alternative lengthening of telomeres. Methods (2017) 114:54–59 | NW Cho, MA Lampson, RA Greenberg | 27491801 | 2017 |
| Confocal Microscopy | SPECTRA X | Imaging of DAPI, Alexa Fluor 488 and Cy3 immunofluorescence using X-Light spinning disk system | Differentiation of control and ALS mutant human iPSCs into functional skeletal muscle cells, a tool for the study of neuromuscular diseases. Stem Cell Res (2016) 17(1):140–147 | J Lenzi, F Pagani, A Rosa et al. | 27318155 | 2016 |
| Confocal Microscopy | SPECTRA X | Excitation of fluorescent protein redox sensors for confocal imaging using Yokogawa CSU-X1 spinning disk system | Redox sensor proteins for highly sensitive direct imaging of intracellular redox state. Biochem Biophys Res Commun (2015) 457(3):242–248 | K Sugiura, T Nagai, T Hisabori et al. | 25592971 | 2015 |
| Confocal Microscopy | SPECTRA X | Excitation of mCherry for confocal imaging of telomere clustering using X-Light spinning disk system | Interchromosomal homology searches drive directional ALT telomere movement and synapsis. Cell (2014) 159(1):108–121 | NW Cho, RL Dilley, RA Greenberg et al. | 25259924 | 2014 |
| Confocal Microscopy | SPECTRA X | Excitation of Rhodamine PE (560 nm) and Alexa Fluor 647 (628 nm) for confocal imaging using X-Light spinning disk system | Phosphoinositides and membrane curvature switch the mode of actin polymerization via selective recruitment of toca-1 and Snx9. Proc Natl Acad Sci U S A (2013) 110(18):7193–7198 | JL Gallop, A Walrant, MW Kirschner et al. | 23589871 | 2013 |
| Fluorescence in Situ Hybridization (FISH) | SOLA SE | Excitation of Hoechst 33342 nuclear stain and PlatinumBright 550-labeled centromere probes | Micro fluorescence in situ hybridization (μFISH) for spatially multiplexed analysis of a cell monolayer. Biomed Microdevices (2016) 18(2):40 | D Huber, J Autebert, GV Kaigala | 27138995 | 2016 |
| Fluorescence in Situ Hybridization (FISH) | SOLA SE | Excitation of JF549 and JF646 fluorophores on dCas9/sgRNA hybridization probes | CASFISH: CRISPR/Cas9-mediated in situ labeling of genomic loci in fixed cells. Proc Natl Acad Sci U S A (2015) 112(38):11870–11875 | W Deng, X Shi, RH Singer et al. | 26324940 | 2015 |
| Fluorescence in Situ Hybridization (FISH) | SPECTRA X | Excitation of Cy3 labeled RNA probes for single molecule FISH detection | Single-gene dual-color reporter cell line to analyze RNA synthesis in vivo. Methods (2016) 103:77–85 | M Palangat, DR Larson | 27068658 | 2016 |
| FRET | SPECTRA X | Excitation of EKAR2G CFP/YFP FRET biosensor for imaging of ERK activation | Frequency modulation of ERK activation dynamics rewires cell fate. Mol Syst Biol (2015) 11(11):838 | H Ryu, M Chung, O Pertz et al. | 26613961 | 2015 |
| FRET | SOLA SE | Excitation of donor (mTurquoise) and acceptor (NeonGreen) fluorescent proteins in a vinculin activation FRET biosensor. | Molecular mechanism of vinculin activation and nano-scale spatial organization in focal adhesions. Nat Cell Biol (2015) 17(7):880–892 | LB Case, MA Baird, CM Waterman et al | 26053221 | 2015 |
| FRET | SPECTRA | Excitation of GFP (donor) at 472 nm and mCherry (acceptor) at 543 nm | A sensitized emission based calibration of FRET efficiency for probing the architecture of macromolecular machines. Cell Mol Bioeng (2013) 6(4):369–382 | A Joglekar, R Chen, J Lawrimore | 24319499 | 2013 |
| FRET | SPECTRA | Excitation of cpCerulean (donor) at 433 nm and cpVenus (acceptor) at 515 nm | Orientation-based FRET sensor for real-time imaging of cellular forces. J Cell Sci (2012) 125(3):743–750 | F Meng, F Sachs | 22389408 | 2012 |
| Genomic Analysis | SPECTRA X | Excitation of EvaGreen dye for high resolution melting (dHRM) analysis of bacterial pathogen DNA sequences | Massively parallel digital high resolution melt for rapid and absolutely quantitative sequence profiling. Sci Rep. (2017) 7:42326 | DO Velez, H Mack, SI Fraley et al. | 28176860 | 2017 |
| Genomic Analysis | SPECTRA X | Real-time detection of multiple displacement DNA amplification (MDA) reactions in hydrogels | Virtual microfluidics for digital quantification and single-cell sequencing. Nat Methods (2016) 13(9):759–762 | L Xu, IL Brito, PC Blainey et al. | 27479330 | 2016 |
| High-Content Screening | SPECTRA X | Imaging of binucleated cells (mitotic index) for assessment of cell cycle progression | Cdk1 activity acts as a quantitative platform for coordinating cell cycle progression with periodic transcription. Nat Commun (2016) 7:11161 | G Banyai, F Baïdi, Z Szilagyi et al. | 27045731 | 2016 |
| High-Content Screening | SOLA SE | 5 channel fluorescence excitation for image-based assessment of RNAi-induced phenotypes. | Morphological profiles of RNAi-induced gene knockdown are highly reproducible but dominated by seed effects. PLoS ONE (2015) 10(7):e013137. | S Singh, X Wu, AE Carpenter et al. | 26197079 | 2015 |
| High-Content Screening | SOLA SE | 5 channel (DAPI/FITC/Cy3/Texas Red/Cy5) excitation for cellular morphology profiling | Cell Painting, a high-content image-based assay for morphological profiling using multiplexed fluorescent dyes. Nat Protoc (2016) 11(9):1757–1774 | MA Bray, S Singh, AE Carpenter et al. | 27560178 | 2016 |
| High-Content Screening | SPECTRA X | Excitation of Hoechst 33342 (379 nm) and deglycosylation-dependent Venus YFP (485 nm) for identification of N-glycanase 1 deficient fibroblasts | A congenital disorder of deglycosylation: Biochemical characterization of N-glycanase 1 deficiency in patient fibroblasts. Glycobiology (2015) 25(8):836–844 | P He, JE Grotzke, HH Freeze et al. | 25900930 | 2015 |
| High-Content Screening | SPECTRA X | Excitation of Alexa Fluor 488 and Alexa Fluor 568 transferrin conjugates for quantitative imaging of cellular transferrin content | Quantitative analysis of transferrin cycling by automated fluorescence microscopy. Methods Mol Biol (2015) 1270:365–378 | DT Hirschmann, CA Kasper, M Spiess | 25702129 | 2015 |
| High-Content Screening | SPECTRA X | Excitation of Hoechst 33342 (377 nm), YOYO-1 (482 nm), MitoTracker Orange (543 nm) and DyLight 649 (650 nm) in 4-color multiplex hepatotoxicity assay | A micropatterned hepatocyte coculture model for assessment of liver toxicity using high-content imaging analysis. Assay Drug Dev Technol (2014) 12(1):16–27 | OJ Trask, A Moore, EL LeCluyse | 24444127 | 2014 |
| High-Content Screening | SPECTRA X | Excitation of GFP, YFP and mCherry for automated bacterial morphology classification | MicrobeJ, a tool for high throughput bacterial cell detection and quantitative analysis. Nat Microbiol (2016) 1(7):16077 | A Ducret, EM Quardokus, YV Brun | 27572972 | 2016 |
| Immunofluorescence Microscopy | SPECTRA | High-resolution imaging of chromatin organization combining multiplexed immunostaining, fluorescence in situ hybridization (FISH) and array tomography (AT) | A high-resolution imaging approach to investigate chromatin architecture in complex tissues. Cell (2015) 163(1):246–255 | MW Linhoff, SK Garg, G Mandel | 26406379 | 2015 |
| Immunofluorescence Microscopy | SOLA SE | (1) Excitation of Alexa Fluor 488, Alexa Fluor 568 and Alexa Fluor 647 labels. (2) Photoconversion of mEos2 (395 nm) | Talin determines the nanoscale architecture of focal adhesions. Proc Natl Acad Sci U S A (2015) 112(35):E4864–E4873 | J Liu, Y Wang, P Kanchanawong et al. | 26283369 | 2015 |
| Immunofluorescence Microscopy | SOLA SE | Visualization of dual immunofluorescence labeling in mouse pancreatic islets | Adipsin Is an adipokine that Improves beta cell function in diabetes. Cell (2014) 158(1):41–53 | JC Lo, S Ljubicic, BM Spiegelman et al. | 24995977 | 2014 |
| Immunofluorescence Microscopy | SOLA SE | Fluorescence immunohistochemical visualization of biomarkers in stem cell-derived intestinal epithelia | Cloning and variation of ground state intestinal stem cells. Nature (2015) 522:173–178 | X Wang, Y Yamamoto, W Xian et al. | 26040716 | 2015 |
| Hyperspectral Imaging | SPECTRA | In-vivo multispectral imaging for detection of stomach carcinoma | In-vivo multispectral video endoscopy towards in-vivo hyperspectral video endoscopy. J Biophotonics (2017) 10(4):553–564 | M Hohmann, R Kanawade, H Albrecht et al. | 27403639 | 2017 |
| Hyperspectral Imaging | SPECTRA X | Integrated quantitative diffuse reflectance imaging and hyperspectral fluorescence imaging of protoporphyrin IX (PpIX) | Quantitative spatial frequency fluorescence imaging in the sub-diffusive domain for image-guided glioma resection. Biomed Opt Express (2015) 6(12):4923–4933 | M Sibai, I Veilleux, BC Wilson et al. | 26713206 | 2015 |
| Intravital Imaging | SPECTRA X | Time-lapse fluorescence ratio imaging of HyPer-transgenic zebrafish using blue (438/57 nm) and cyan (475/28 nm) excitation | Image-based measurement of H2O2 reaction-diffusion in wounded zebrafish larvae. Biophys J (2017) 112(9):2011–2018 | M Jelcic, B Enyedi, P Niethammer et al. | 28494970 | 2017 |
| Intravital Imaging | SPECTRA X | Bright-field and autofluorescence imaging of C. elegans every 3 hours for up to 16 days | Extended twilight among isogenic C. elegans causes a disproportionate scaling between lifespan and health. Cell Syst (2016) 3(4):333–345 | WB Zhang, DB Sinha, Z Pincus et al. | 27720632 | 2016 |
| Intravital Imaging | SPECTRA X | Intraoperative indocyanine green (ICG) fluorescence imaging of tumors | Quantification of tumor fluorescence during intraoperative optical cancer imaging. Sci Rep (2015) 5:16208 | RP Judy, JJ Keating, S Singhal et al. | 26563091 | 2015 |
| Intravital Imaging | SPECTRA X | Excitation of Alexa Fluor 488– and Alexa Fluor 647–labeled antibodies for imaging fibrin generation and platelet accumulation in mice | Novel mouse hemostasis model for real-time determination of bleeding time and hemostatic plug composition. J Thromb Haemost (2015) 13(3):417–425 | TM Getz, R Piatt, W Bergmeier et al. | 25442192 | 2015 |
| Live Cell Microscopy | SPECTRA X | Excitation of green fluorescent (GCaMP6m) and red fluorescent (R-CaMP2) genetically encoded calcium indicators in rat hippocampal neurons | Simultaneous fast measurement of circuit dynamics at multiple sites across the mammalian brain. Nat Methods (2016) 13(4):325–328 | CK Kim, SJ Yang, K Deisseroth et al. | 26878381 | 2016 |
| Live Cell Microscopy | SPECTRA X | Time-lapse imaging of microtubule dynamics in LLC-PK1 porcine epithelial cells stably expressing EGFP–α-tubulin or EB1–EGFP | Mechanisms of kinetic stabilization by the drugs paclitaxel and vinblastine. Mol Biol Cell (2017) 28(9):1238–1257 | BT Castle, S McCubbin, DJ Odde et al. | 28298489 | 2017 |
| Live Cell Microscopy | SPECTRA X | Fluorescence ratio imaging of H2O2 sensor HyPer with alternating 100 ms excitation pulses at 475/28 nm and 438/24 nm | Aquaporins facilitate hydrogen peroxide entry into guard cells to mediate ABA- and pathogen-triggered stomatal closure. Proc Natl Acad Sci U S A (2017) 114(34):9200–9205 | O Rodrigues, G Reshetnyak, L Verdoucq et al. | 28784763 | 2017 |
| Live Cell Microscopy | SPECTRA X | Excitation of CFP (434/17 nm), GFP (488/10 nm) and mCherry (560/25 nm) | Correlative live cell and super resolution imaging of autophagosome formation. Methods Enzymol (2017) 587:1–20 | SA Walker, E Karanasios, NT Ktistakis | 28253951 | 2017 |
| Live Cell Microscopy | SOLA SE 365 | Imaging of FRET biosensors for pyruvate kinase activity and ATP/ADP and endogenous NADPH and flavins | Phase analysis of metabolic oscillations and membrane potential in pancreatic islet β-cells. Biophys J (2016) 110(3):691–699 | MJ Merrins, C Poudel, LS Satin et al. | 26840733 | 2016 |
| Live Cell Microscopy | SPECTRA X | Imaging origins of replication loci (oriC) in E. coli expressing LacI-tagGFP | CRISPR-mediated control of the bacterial initiation of replication. Nucleic Acids Res (2016) 44(8):3801–3810 | J Wiktor, C Lesterlin, C Dekker et al. | 27036863 | 2016 |
| Live Cell Microscopy | SOLA SE | Excitation of GFP-ALADIN and mCherry–α-tubulin expressed in Drosophila S2 cells | The nucleoporin ALADIN regulates Aurora A localization to ensure robust mitotic spindle formation. Mol Biol Cell (2015) 26(19):3424–3438 | S Carvalhal, SA Ribeiro, ER Griffis et al. | 26246606 | 2015 |
| Live Cell Microscopy | SPECTRA X | Excitation of GFP and RFP for imaging of mitochondrial fusion | Mesenchymal stem cells use extracellular vesicles to outsource mitophagy and shuttle microRNAs. Nat Commun (2015) 6:8472 | DG Phinney, M Di Giuseppe, LA Ortiz et al. | 26442449 | 2015 |
| Live Cell Microscopy | SPECTRA | Excitation of luminescent temperature sensor europium (III) thenoyltrifluoroacetonate (Eu-TTA) at 377 nm | Triggering of high-speed neurite outgrowth using an optical microheater. Sci Rep (2015) 5:16611 | K Oyama, V Zeeb, S Ishiwata et al. | 26568288 | 2015 |
| Live Cell Microscopy | SPECTRA X | Imaging of photoinduced lysosome damage in C. neoformans–infected bone marrow–derived macrophages | Cryptococcus neoformans-induced macrophage lysosome damage crucially contributes to fungal virulence. J Immunol (2015) 194(5):2219–2231 | MJ Davis, AJ Eastman, MA Olszewski et al. | 25637026 | 2015 |
| Live Cell Microscopy | SPECTRA X | Excitation of PercevalHR ATP/ADP sensor at 438 nm and 482 nm | Imaging changes in the cytosolic ATP-to-ADP ratio. Methods Enzymol (2014) 547:355–371 | M Tantama, G Yellen | 25416365 | 2014 |
| Live Cell Microscopy | SOLA SE | 24-hour time-lapse imaging of promoter-dependent GFP expression in E. coli | Quorum-sensing crosstalk-driven synthetic circuits: from unimodality to trimodality. Chem Biol. (2014) 21(12):1629–1638 | F Wu, DJ Menn, X Wang | 25455858 | 2014 |
| Live Cell Microscopy | SPECTRA | High-speed (50 fps) imaging of GFP (ex = 470 nm) and mKate2 (ex = 555 nm) transgenic C. elegans | Non-microfluidic methods for imaging live C. elegans. Methods (2014) 68(3):542–547 | CJ Luke, JZ Niehaus, SC Watkins et al. | 24836996 | 2014 |
| Live Cell Microscopy | SPECTRA | Excitation of GFP and mCherry in cultured hippocampal neurons | Axonal actin in action: Imaging actin dynamics in neurons. Methods Cell Biol (2016) 131:91–106 | K Ladt, A Ganguly, S Roy | 26794509 | 2016 |
| Live Cell Microscopy | SPECTRA X | Calcium imaging in cardiac myocytes using fluo-4 (ex = 470 nm) | Cardiac CD47 Drives Left Ventricular Heart Failure Through Ca2+‐CaMKII‐Regulated Induction of HDAC3. J Am Heart Assoc. (2014) 3(3): e000670 | M Sharifi-Sanjani, AH Shoushtari, JS Isenberg et al. | 24922625 | 2014 |
| Live Cell Microscopy | SOLA SE | Pulsed excitation (450-490 nm) of GCaMP calcium sensor to limit phototoxic damage in live C. elegans nematodes | High-throughput imaging of neuronal activity in Caenorhabditis elegans. Proc Natl Acad Sci U S A (2013) 110(45):E4266-4273 | J Larsch, D Ventimiglia, DR Albrecht et al. | 24145415 | 2013 |
| Live Cell Microscopy | SOLA SE | Imaging of time-dependent GFP (ex = 472 nm) and mCherry (ex = 562 nm) expression in yeast | Engineering of regulated stochastic cell fate determination. Proc Natl Acad Sci U S A (2013) 110(26):10610–10615 | M Wu, RQ Su, X Wang et al. | 23754391 | 2013 |
| Live Cell Microscopy | SOLA SE | imaging GFP-labeled endogenous β-actin mRNA in cultured neurons | Imaging single mRNA dynamics in live neurons and brains. Methods Enzymol (2016) 572:51–64 | HC Moon, HY Park | 27241749 | 2016 |
| Materials Science | SOLA SM | Focal plane shifted time-lapse imaging of water droplets on superhydrophobic surfaces | Focal plane shift imaging for the analysis of dynamic wetting processes. ACS Nano (2016) 10(9):8223–8232 | H Cha, JM Chun, N Miljkovic et al. | 27447844 | 2016 |
| Materials Science | SOLA SE | Imaging of single quantum dots (QDs) on silver plasmonic resonators | Wedge waveguides and resonators for quantum plasmonics. Nano Lett (2015) 15(9):6267–6275 | SJ Kress, FV Antolinez, DJ Norris et al. | 26284499 | 2015 |
| Microfluidics | SOLA SE | Microfluidics-assisted cell screening (MACS) of E. coli | Mechanical slowing-down of cytoplasmic diffusion allows in vivo counting of proteins in individual cells. Nat Commun (2016) 7:11641 | B Okumus, D Landgraf, J Paulsson et al. | 27189321 | 2016 |
| Microfluidics | SOLA SE | Photoactivation of MNI-caged glutamate | One-photon and two-photon stimulation of neurons in a microfluidic culture system. Lab Chip (2016) 16(9):1684–1690 | JM Jang, J Lee W Jung et al. | 27053163 | 2016 |
| Microfluidics | SOLA SE | Imaging micropattered goat anti-IgG deposition on IgG-coated polystyrene surfaces | Convection-enhanced biopatterning with recirculation of hydrodynamically confined nanoliter volumes of reagents. Anal Chem (2016) 88(6):3235–3242 | J Autebert, JF Cors, G V Kaigala et al. | 26837532 | 2016 |
| Microfluidics | SPECTRA X | Evaluating the color of thermochromic liquid crystal (TLC) fluid flow tracers | Simultaneous three-dimensional temperature and velocity field measurements using astigmatic imaging of non-encapsulated thermo-liquid crystal (TLC) particles. Lab Chip (2015) 15:660–663 | R Segura, M Rossi, CJ Kähler et al. | 25431101 | 2015 |
| Microfluidics | SPECTRA X | 470 nm excitation of GFP for time-lapse imaging of cells in microfluidic networks | Reconfigurable microfluidic hanging drop network for multi-tissue interaction and analysis. Nat Commun (2014) 5:4250 | O Frey, PM Misun, A Hierlemann et al. | 24977495 | 2014 |
| Microfluidics | SOLA SM | Imaging of binary micropatterns of Alexa Fluor 488– and Alexa Fluor 555–labeled antibodies deposited on glass surfaces | Hierarchical hydrodynamic flow confinement: efficient use and retrieval of chemicals for microscale chemistry on surfaces. Langmuir (2014) 30(12):3640–3645 | J Autebert, A Kashyap, GV Kaigala et al. | 24625080 | 2014 |
| Microscopy Technique Development | SPECTRA X | Acquisition of whole slide images (WSIs) for validation of BaSiC shading correction method | A BaSiC tool for background and shading correction of optical microscopy images. Nat Commun (2017) 8:14836 | T Peng, K Thorn, N Navab et al. | 28594001 | 2017 |
| Microscopy Technique Development | SPECTRA X | Validation of expansion microscopy (ExM) on cells and tissues with multicolor immunofluorescence and fluorescent protein labeling | Protein-retention expansion microscopy of cells and tissues labeled using standard fluorescent proteins and antibodies. Nat Biotechnol (2016) 34(9):987–992 | PW Tillberg, F Chen, ES Boyden et al. | 27376584 | 2016 |
| Microscopy Technique Development | SPECTRA X | Validation of a computational method for illumination uniformity correction | CIDRE: an illumination-correction method for optical microscopy. Nat Methods (2015) 12(5):404–406 | K Smith, Y Li, P Horvath et al. | 25775044 | 2015 |
| Multimode Imaging | SPECTRA | Optimization of GFP fluorescence in dehydrated resin sections for correlative light and electron microscopy | Correlative and integrated light and electron microscopy of in-resin GFP fluorescence, used to localise diacylglycerol in mammalian cells. Ultramicroscopy (2014) 143:3–14 | CJ Peddie, K Blight, LM Collinson et al. | 24637200 | 2014 |
| Multimode Imaging | SOLA SE | Cryogenic fluorescence microscopy of high-pressure frozen cells on sapphire disks | triCLEM: Combining high-precision, room temperature CLEM with cryo-fluorescence microscopy to identify very rare events. Methods Cell Biol (2017) 140:303–320 | NR Ader, W Kukulski | 28528638 | 2017 |
| Optogenetics | SPECTRA | Stimulation (ChR2) and inhibition (Arch-T) of transmembrane potassium flux in developing Xenopus embryos | Bioelectric signalling via potassium channels: a mechanism for craniofacial dysmorphogenesis in KCNJ2-associated Andersen-Tawil Syndrome. J Physiol (2016) 594(12):3245–3270 | DS Adams, SG Uzel, M Levin et al. | 26864374 | 2016 |
| Optogenetics | SPECTRA X | (1) Activation of photosensitive G protein-coupled receptor melanopsin (470 nm). (2) Excitation of X-rhod-1 Ca2+ indicator (570 nm) | Optogenetic control of calcium oscillation waveform defines NFAT as an Integrator of calcium load. Cell Syst (2016) 2(4):283–288 | P Hannanta-Anan, BY Chow | 27135540 | 2016 |
| Optogenetics | SPECTRA X | Activation (475/28 nm; 8 mW/mm2) and deactivation (632/22 nm; 5 mW/mm2) of step-function opsin (SSFO) and the step-function inhibitory opsin SwiChR++ | Modulation of prefrontal cortex excitation/inhibition balance rescues social behavior in CNTNAP2-deficient mice. Sci Transl Med (2017) 9:401 | A Selimbeyoglu, CK Kim, K Deisseroth et al. | 28768803 | 2017 |
| Optogenetics | SOLA SE | (1) Stimulation of ChR2 (470 nm) and Chrimson (615 nm) photoproteins. (2) Excitation of GCaMP3 Ca2+ sensor. | Feedback from network states generates variability in a probabilistic olfactory circuit. Cell (2015) 161(2):215–227. | A Gordus, N Pokala, CI Bargmann et al. | 25772698 | 2015 |
| Optogenetics | SPECTRA X | Optical activation of ChR2-expressing neurons (475/28 nm; 10 ms light pulses at 19 mW/mm2) | A sexually dimorphic hypothalamic circuit controls maternal care and oxytocin secretion. Nature (2015) 525(7570):519–522 | N Scott, M Prigge, T Kimchi et al. | 26375004 | 2015 |
| Optogenetics | SPECTRA X | Identification of melanopsin-based responses in photosensitive retinal ganglion cells using alternating blue (438 nm) and yellow (575 nm) stimuli | Melanopsin-derived visual responses under light adapted conditions in the mouse dLGN. PLoS ONE (2015) 10(3):e0123424 | KE Davis, CG Eleftheriou, RJ Lucas et al. | 25822371 | 2015 |
| Optogenetics | SOLA SE | Photoactivation of CRY2-CIB1 heterodimerization | Optogenetic control of molecular motors and organelle distributions in cells. Chem Biol (2015) 22(5):671–682 | L Duan, D Che, B Cui et al. | 25963241 | 2015 |
| Optogenetics | SOLA SE | Transient (~100 ms) stimulation of ChR2 (460 –490 nm) in zebrafish hair cells | Optical stimulation of zebrafish hair cells expressing channelrhodopsin-2. PLoS ONE (2014) 9(5): e96641 | BD Monesson-Olson, J Browning-Kamins, JG Trapani et al. | 24791934 | 2014 |
| Optogenetics | SPECTRA | Stimulation (ChR2, 475 nm) and inhibition (Arch-T, 549 nm) of melanin-concentrating hormone (MCH) producing neurons | Optogenetic manipulation of activity and temporally controlled cell-specific ablation reveal a role for MCH neurons in sleep/wake regulation. J Neurosci (2014) 34(20):6896–6909 | T Tsunematsu, T Ueno, A Yamanaka et al. | 24828644 | 2014 |
| Optogenetics | SPECTRA X | Activation (475 nm) and deactivation (632 nm) of bi-stable light-activated chloride channel SwiChR | Structure-guided transformation of channelrhodopsin into a light-activated chloride channel. Science (2014) 344(6182):420–424 | A Berndt, SY Lee, K Deisseroth et al. | 24763591 | 2014 |
| Optogenetics | SPECTRA X | Stimulation of light-activated chloride channel ChloC (476 nm) | Conversion of channelrhodopsin into a light-gated chloride channel. Science (2014) 344(6182):409–412 | J Wietek, JS Wiegert, P Hegemann et al. | 24674867 | 2014 |
| Optogenetics | SPECTRA X | Activation of ChR2 and NpHR with cyan (475 nm) and yellow (586 nm) light respectively in mouse dorsal root ganglion neurons | Virally mediated optogenetic excitation and inhibition of pain in freely moving nontransgenic mice. Nature Biotech (2014) 32(3):274–278 | SM Iyer, KL Montgomery, SL Delp et al. | 24531797 | 2014 |
| Optogenetics | SPECTRA | Transient (1–200 ms) stimulation of ChR2 (475 nm) in rat cortical neurons | Role of electrical activity in horizontal axon growth in the developing cortex: a time-lapse study using optogenetic stimulation. PLoS ONE (2013) 8(12):e82954 | O Malyshevskaya, Y Shiraishi, N Yamamoto et al. | 24376616 | 2013 |
| Optogenetics | SPECTRA | Optogenetic inhibition of Arch-T expressing neurons (542 nm) | Long-lasting silencing of orexin/hypocretin neurons using archaerhodopsin induces slow-wave sleep in mice. Behav Brain Res (2013) 255:64–74 | T Tsunematsu, S Tabuchi, A Yamanaka et al. | 23707248 | 2013 |
| Photochemistry | SPECTRA | 75 mW/cm2 illumination of zinc porphyrin MPZnP photosensitizer at 445 nm | Growth and post-deposition treatments of SrTiO3 films for dye-sensitized photoelectrosynthesis cell applications. ACS Appl Mater Interfaces (2016) 8(19):12282–12290 | RW Call, L Alibabaei, R Lopez et al. | 27128813 | 2016 |
| Photochemistry | SPECTRA | Irradiation of ruthenium polypyridyl sensitizer at 445 nm for photoelectrochemical water splitting | Visible photoelectrochemical water splitting into H2 and O2 in a dye-sensitized photoelectrosynthesis cell. Proc Natl Acad Sci U S A (2015) 112(19):5899–5902 | L Alibabaei, BD Sherman, TJ Meyer et al. | 25918426 | 2015 |